CHEMOKINE RECEPTOR CXCR4 EXPRESSION AND MICROVESSELS DENSITY IN MALIGNANT TUMORS OF THE BREAST OF PATIENTS WITH SIGNS OF METABOLIC SYNDROME

Iurchenko N.P., Nesina I.P., Breeva O.V., Chekhun V.F., Buchynska L.G.

• R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

Aim: to evaluate CXCR4, S18-2 expression and microvessel density (MVD) in malignant tumors of the breast of different molecular subtypes, depending on the presence in patients of such signs of metabolic syndrome (MS) as obesity and hypertension (AH). Object and methods: samples of surgical material and peripheral blood serum (PBS) for 65 breast cancer (ВС) patients (stage I–II) of the tumor process, with different molecular subtypes: luminal A (37 cases), luminal B (14 cases) and basal (10 cases). The average age of patients is 58.0 ± 1.9 years. Methods: clinical, morphological, immunohistochemical (IHC), enzyme-linked immunoaassay (ELISA), statistical. Results: the highest expression of the chemokine receptor CXCR4 and MVD was found in breast tumors with the molecular subtype luminal B (51.6 ± 4.2% and 13,5±2,3 vessels/mm² respectively). For luminal A and basal subtypes, these indicators decreased: CXCR4 (49.9 ± 2.8% and 41.4 ± 4.0% respectively) and MVD (5,1 ± 1,3 vessels/mm², p <0.05 and 8,0 ± 1,7 vessels/mm² respectively). Increased expression of CXCR4 (55.8 ± 2.9%) and S18-2 (10.1 ± 1.1%) and MVD (8.0 ± 1.7 vessels mm²) was detected in the tumor tissue of the luminal A subtype of patients with signs MS compared to the expression of these parameters in tumors of patients without signs of MS (48.7 ± 2.4%, 5.2 ± 0.3%, p < 0.05 and 5.3 ± 1.8 vessels/mm² respectively). Increased expression of CXCR4 (51.6 ± 2.8%) and S18-2 (11.9 ± 1.2%) and MVD (7.4 ± 2.0 vessels/mm²) were determined in tumors of ВС patients with cancer metastases compared with the values of these parameters in tumors of patients without metastases: 43.4 ± 2.3%; 4.6 ± 0.8%, p <0.05 and 5.3 ± 1.8 vessels/mm² respectively. Conclusions: increased expression of the chemokine receptor CXCR4, mitochondrial ribosomal protein S18-2 and MVD in breast tumor cells is associated with both the molecular subtypes of breast cancer and the presence of obesity and hypertension in patients and is associated with the occurrence of patients with metastases, which may indicate the prognostic value of investigated markers for detectable markers the course of the disease.