GENE EXPRESSION OF POLYAMINE METABOLISM PROTEINS IN HUMAN PROSTATE CANCER AND BENIGN PROSTATIC HYPERPLASIA

Bentrad V.V.¹, Zaletok S.P.¹, Klenov O.О.¹, Vitruk Yu.V.², Stakhovsky Е.О.²

• ¹R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NAS of Ukraine
• ²National Cancer Institute, Kyiv, Ukraine

Prostate cancer and benign prostatic hyperplasia are currently the most common oncopathologies of older men. Recent studies suggest that the metabolism of polyamines in prostate tumors differs in tumors of other locations. Objective: to investigate the gene expression of polyamines biosynthesis enzymes — ornithine decarboxylase and spermine synthase — and, spermine catabolism enzyme — acetyltransferase in malignant and benign prostate cancer cells. Object and methods: the study was performed on 25 postoperative samples of tumors from patients with prostate cancer (PC) and benign prostatic hyperplasia (BHP): 7 samples of benign and 18 samples of malignant tumors. The gene expression was determined using biochemical, immunological and molecular methods. Results: The decreased level of ornithine decarboxylase expression in malignant prostate tumors was determined compared with benign tumors. It is shown that the level of spermine synthase in malignant prostate tumors, in contrast to benign, detected only in trace amounts. However, the level of expression of spermine acetyltransferase in malignant prostate tumors was higher compared with benign tumors and increased with disease progression. Conclusion: the results indicate the diversity of changes in polyamine metabolism in benign and malignant prostate cancer tumors. The obtained data can be further used for accurate diagnosis of prostate cancer and for the search of targeted anticancer drugs.