BLOOD AND URINE POLYAMINES AS NEW DIAGNOSTIC MARKERS OF PROSTATE CANCER
Zaletok S.P., Klenov O.О., Gogol S.V., Bentrad V.V., Stakhovsky Е.О., Vitruk Yu.V., Grechko B.O.
Prostate cancer (PC) is one of the prevalent malignancies in men of 50 and more years old. Currently, the commonly used marker for PC screening is PSA (prostate specific antigen). But PSA test is not enough sensitive and specific. Therefore, it is extremely important to find and develop new markers, in particular those that could be identified in the biological fluids of patients. Polyamines (PAs) deserve special attention as potential markers for differential diagnosis of PC. Aim: to specify level of spermine and other PA in blood and urine of the patients with PC, benign prostate tumors (BPT) and practically healthy men and evaluate efficacy of PA as extratumoral PC markers. Object and methods: level of PA (putrescine, spermidine, spermine and their acetylated forms) were measured in blood and urine of 120 PC patients, 30 BPT patients and 30 practically healthy men (control group). PA content was measured by HPLC and ELISA methods. Statistical data treatment was done using standard licensed computer programs STATISTICA 6.0, Microsoft Ехсеl, ANOVA «Statistica». Results: spermine level in the PC patients’ blood was shown to be significantly lower versus both BPT patients and practically healthy men. During the disease progress (from indolent to aggressive cancer), both blood spermidine level and spermidine/spermine ratio increase. In blood of PC patients, in contrary to BPT patients and healthy men, N1,N12-diacetylspermine was found. In PC patients urine, spermine level was 34 times lower than in healthy men and 13 times lower than in BPT patients. Conclusion: it is expedient to include PA measurement into the list of methods using to examine the patients with the prostate tumors. This would be useful to refine differential diagnosis of the prostate tumors and promote individualized therapy.
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