EXPERIMENTAL EVALUATION OF INFLUENCE OF IMPLANTATION MATERIALS BASED ON CALCIUM PHOSPHATES ON MOLECULAR-BIOLOGICAL CHARACTERISTICS OF NORMAL AND MALIGNANT TRANSFORMED CELLS OF HORMONE-DEPENDENT TUMORS

Lukianova N.Yu., Zadvornyi T.V., Borikun T.V., Chekhun V.F.

• RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine

Aim: to study the effect of implant materials (IM) based on calcium phosphates on the molecular biological characteristics of normal and malignantly transformed hormone-dependent cells in the in vitro system. Objects and methods: cells of normal human fibroblasts, prostate cancer lines (LNCaP and DU-145) and breast cancer (MCF-7, MDA-MB-231) were cultured with IM at a dose corresponding to IC30 (100 μg/ml) during 48 hours. Expression levels of molecular markers were determined by immunocytochemical method. MicroRNA levels were assessed by quantitative PCR. Analysis of functional relationships and interactions between experimental molecules was performed using the STRING v.11.0 algorithm. Statistical analysis of the results was performed using the Statistics 6.0. Software. Results: the analysis of cytomorphological features of cells exposed to IM revealed a unidirectional change that indicate an increase in their adhesive properties, as evidenced by the increase in expression of E- and N-cadherin in cells of human breast and prostate cancer cells. It was found that under the action of the tested substance in the cancer cells is an increase in the expression of oncogenic miRNA-221, which reduces the level of steroid hormone receptors. It was determined that IM does not affect the receptor status, the expression of adhesion molecules and Ki-67 in cells of normal fibroblasts. Conclusions: the cultivation of malignantly transformed cells of the human breast cancer and prostate cancer with IM leads to a decrease in their invasive properties due to changes in cytoarchitectonics and adhesive characteristics. The established changes in the posttranscriptional regulation of proteins associated with receptor status in the cells of breast and prostate cancer under the influence of phosphate-based IM require further research.