EXPRESSION OF PROTEINS OF POLYAMINE METABOLISM FERMENTS IN HUMAN PROSTATE MALIGNANT TUMORS

Gogol S.V.

• R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

Enzymes involved in polyamine exchange (PA) play an important role in regulating the growth, differentiation, and programmed cell death processes. The study of these enzymes in prostate cancer (PC) tissue is appropriate both for deepening know­ledge about the pathogenesis of the disease and for eva­luating the possibility of using PA metabolism as additional criteria for predicting the course of PC. Objective: to study the expression level of PA metabolites (arginase (Arg I), ornithine decarboxylase (ODC), polyamino oxi­dase (PAO)) in PC cells depending on the stage of the disease. Object and methods: the expression levels of Arg I, ODC, and PAO proteins in 35 samples (ope­rational material) of PC stage I–IV were investigated using biochemical, immunological, molecular me­thods, as well as mathematical processing of results using a Total­Lab computer program. Results: reduced levels of expression of Arg I and ODK proteins in PC cells as disease progressed. The highest levels of Arg I and ODC expression were detected in samples of PC stage I, the lowest (by 34.2 and 50.7%, respectively) in stage IV tumors. On the contrary, PAO expression levels in PC cells with increasing stage of the disease are increasing: in patients with stage IV, this indicator increased by 42.6% compared with stage I. Conclusion: determination of expression levels of proteins of polyamine metabolism enzymes in PC can be proposed as additional criteria for specifying the diagnosis and prognosis of PC.