DETERMINATION OF CORRELATION BETWEEN THE STATE OF PRO- AND ANTIOXIDANT PROCESSES IN THE BLOOD OF PATIENTS WITH PROSTATE CANCER AND CHROMOSOMAL INSTABILITY OF BLOOD LYMPHOCYTES

Glavin O.A., Domina E.A., Makovetska L.I., Mikhailenko V.M.

Objective: to investigate the intensity of pro- and antioxidant processes in blood and the degree of chromosomal instability in peripheral blood cells of patients with prostate cancer (PC) before and after radiotherapy. Object and methods: research was performed on blood of 31 patients with PC (experimental group) and 31 healthy donors (control group) with the use of biochemical, cytogenetic and statistical me­thods. The Fe²⁺ induced production of reactive oxygen species (ROS), the content of sulfhydryl groups of proteins and peptides (SH-groups) and malonic dialdehyde (MDA) in blood plasma; the activity of the catalase in blood; the total production of free radical compounds (FRC), the number of spontaneous (G₀-assay) and radiation induced chromosome aberrations (G₂-­assay) in the peripheral blood lymphocytes (PBL) were determined. Results: in patients with PC, the le­vel of ROS pro­ducts was increased by 1.55 times but the activity of catalase, the concentration of SH-groups and MDA level were reduced by 1.45; 1.24 and 1.12 times, respectively. The number of spontaneous chromosomal aberrations in the PBL exceeded the average population level by 2.84 times. Reduced catalase activity correlated with the size of the primary tumor and the decrease in the content of SH-groups. The level of SH-groups correlated with the amount of spontaneous chromosomal aberrations. The inverse correlation between the individual radiosensitivity of patients (G₂-assay) and the production of FR in the PBL after the first fraction of therapeutic irradiation was revealed. Conclusion: the obtained results suggest the essential role of oxidative stress in the formation of chromosomal instability of normal cells of patients with PC.