Yu.V. Yanish1, M.P. Prylutskyi1, O.K. Voronina2, S.P. Zaletok1
1 R.E. Kavetskyi Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Sciences of Ukraine,
2 T.G. Shevtchenko National university, Kyiv, Ukraine


Summary. Aim: to investigate the inhibitory effect of spermine (Spn) and the modifying effect of aminoguanidine (AG) on the structural and functional characteristics of human prostate cancer cells of the LNCaP line, depending on the mode of their use. Object and methods: studies were conducted in vitro on human prostate cancer (PC) cells of the hormone-dependent LNCaP line. Cell survival was determined by the trypan blue exclusion. The concentration of Spn used in the study was 1.5 and 5.0 mM, and AG in combination with Spn 1.5 mM. Changes in the morphology of LNCaP cells were evaluated under microscopr on the fixed cytological specimens of cells grown on coverslips and stained with hematoxylin and eosin. Results: Spn at a concentration of 1.5 mM exhibites a pronounced inhibitory effect on the growth of LNCaP cells reducing their survival. Cells die at a Spn concentration of 5.0 mM. The presence of AG (1.5 mM) in the culture medium contributes to a significant recovery of cell survival. Conclusions: the increase in Spn concentration from 1.5 mM to 5.0 mM seems to result in apoptotic death of LNCaP cells. In sublethal concentrations, Spn reduces the synthetic and suppresses the mitotic activity of LNCaP cells, while AG shows a certain protective effect.

Keywords: spermine, aminoguanidine, LNCaP cell line, mitosis, apoptosis.



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