THE INFLUENCE OF SPERMINE ON THE SURVIVAL AND ELECTROKINETIC CHARACTERISTICS OF HUMAN PROSTATE CANCER LNCaP CELL LINE
Yanish Yu.V., Prylutskyi M.P., Zaletok S.P.
Aim: study the relationship between the survival of human prostate cancer LNCaP cells, their total surface charge and ζ-potential in the presence of an excess of spermine or putrescine in the nutrient medium and to determine the half-inhibitory concentration IC50 of spermine. Object and methods: the study was conducted using human prostate cancer cells of the LNCaP cell line. Vitality was determined in cell samples, with total number 0.1–1.5•106. Cells were treated with spermine, putrescine, and aminoguanidine at concentration range 0.01–10 mM. The IC50 inhibitory dose was determined using the Quest Graph™ IC50 program (AAT Bioquest, USA). The electrokinetic, or ζ-potential, was calculated according to the equation of M.R. Smolukhovskyi. The density of the total surface charge of the cells was calculated according to the Quincke-Helmholtz equation. Results: the protective properties of aminoguanidine against the inhibitory effect of spermine were demonstrated, if the concentration of the spermine equals or exceeds the IC50. It has been shown that spermine in concentrations of 1.5 mM and higher causes the death of prostate cancer cells in vitro and can be considered as a potential antitumor agent. Conclusion: the main result of this study is the establishment of a clear inhibitory and antiproliferative effect of spermine in certain concentrations in relation to the number of cells. It was shown that alongside with decreasing of cell vitality under the influence of spermine, there was observed a change in the sign of their surface charge from negative to positive.
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