CURRENT STATE OF LABORATORY DIAGNOSIS OF MATURE B-CELL LYMPHOID NEOPLASMS IN UKRAINE

Philchenkov A.A.¹, Zavelevich M.P.¹, Abramenko I.V. ², Polishchuk А.S.¹, Andreieva S.V.³, Korets K.V.⁴, Zinchenkо V.N.⁵, Gluzman D.F.¹

• ¹R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine
• ²National Research Center for Radiation Medicine, NAMS of Ukraine
• ³State Institution «Institute of Haematology and Transfusiology of NAMS of Ukraine»
• ⁴Medical Laboratory «IMMD»
• ⁵City Clinical Hospital No 9, Kyiv, Ukraine

Mature B-cell lymphoid neoplasms constitute a heterogeneous group of diseases with variable clinical presentations ranging from indolent to very aggressive behavior. The revised (2016) WHO classification comprises more than 50 forms and clinicopathological variants of such neoplasms. The representative forms of mature B-cell lymphoid neoplasms (chronic lymphocytic leukemia/small lymphocytic lymphoma, hairy cell leukemia, lymphoplasmacytic lymphoma, marginal zone lymphoma, follicular lymphoma, mantle cell lymphoma, Burkitt lymphoma, diffuse large B-cell lymphoma and multiple myeloma) are characterized focusing on the current state of their diagnosing based on laboratory criteria according to the up-to-date requirements. The diagnosis is based on the detection of typical morphological features and histological and immunohistochemical analysis of trephine biopsies combined with immunophenotyping of the neoplastic cells by flow cytometry. The landscape of various types of chromosomal aberrations and molecular genetic abnormalities in mature B-cell neoplasms and their application in disease diagnosis especially in the cases with atypical laboratory findings are also discussed.