CYTOGENETIC ABERRATIONS IN PATIENTS WITH CHRONIC MYELOID LEUKEMIA AND ITS DETECTION BY FLUORESCENCE IN SITU HYBRIDIZATION (FISH) METHOD

Lukyanova A.S., Valchuk M., Loginsky V., Pieńkowska-Grela B.

The study was performed in 52 patients with suspected chronic myeloid leukemia (CML) to detect BCR/ABL fusion gene and to evaluate the incidence and diagnostic siginificance of different hybridization patterns. BCR/ABL rearrangement was identified in 85% of patients; with typical FISH pattern in 52% and atypical in 48% of the cases. Atypical hybridization resulted from additional fusion signals in 21% of specimens, variant translocations in 11%, ABL gene deletions in 7%, ABL/BCR gene deletions in 5%, an insertion of BCR gene into ABL in 2% and a variant translocation with a concomitant ABL/BCR deletion in 2%.



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