INVESTIGATION OF PROLIFERATIVE ACTIVITY OF CELLS OF RADIOIODINE RESISTANT THYROID PAPILLARY CANCER METASTASES

Zelinska G.V.

Objective: comparative studies of the expression of marker of сеlls’ proliferative activity (the antigen of Ki-67) in punctates of radioiodine-resistant (RIRM), radioiodine-sensitive metastases (RISM) and the gene­ral group of papillary thyroid carcinoma metastases. Object and methods: cytomorphological and imunocytochemical studies using monoclonal antibody against antigen Ki-67 (МІВ-1) were perfomed on the material of fine needle aspiration biopsy of papillary thyroid carcinomas metastases: 29 — were detected before surgery, 38 — were detected in the postoperative period (27 — RIRM, 11 — RISM); as well as on punctates of 4 medul­lary carcinomas, 10 papillary carcinomas (PC) and 10 nodular goiters. Results: the technique of immunocytochemical determination of cells proliferative activity marker in punctates, painted according to the Romanovsky method, was worked out. The highest percentage of Ki-67-positive cells was observed in punctates of medullary carcinoma (7–10%), the diffuse-sclerosing variant of PC (7–12%) and PC associated with autoimmune thyroiditis (6–35%). Based on the results of the Mann — Whitney criterion, significant difference between RIRM and the general population of PC meta­stases, and between RIRM and RISM of papillary thyroid carcinoma wasn’t detected. There was a tendency to increase the content of Ki-67-positive cells in punctates of repeated RIRM of recurrent PC. Conclusion: the absence of statistically significant difference in expression of Ki-67 between the studied groups of papillary thyroid carcinoma metastases was demonstrated. This indicates the independence of radioiodine accumulation capacity from the intensity of the proliferative processes of thyrocytes of papillary metastases. There is a tendency to increase the content of Ki-67-positive cells in punctates of repeated RIRM of recurrent PC.