INDUCTION OF P53-DEPENDENT APOPTOSIS BY IONIZING RADIATION IN LYMPHOID CELLS OF PATIENTS WITH B-CELL CHRONIC LYMPHOCYTIC LEUKEMIA

Zavgorodnyaya А.V., Balan V.I., Kryachouk I.А., Chumak А.А.

The response of peripheral blood lymphocytes from 45 patients with chronic lymphocytic leukemia (CLL) on ionizing radiation (IR) was evaluated. Cell viability and expression of some proteins (p53, p21, MDM2) by Western-blot analysis have been studied. The most of patients (34; 75.6%) showed typical response on IR: death of irradiated cells, induction of p53 and p21, increasing of basal level of MDM2 expression. Eleven patients (24.4%) had different disturbances in the system of p53-dependent apoptosis that might complicate treatment of such CLL patients.