ACRYLAMIDE PHOTOPOLYMERIZATION FOR ANALYTICAL ELECTROPHORESIS

Shlyakhovenko V.O., Samoylenko O.A.

• R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

Aim: To create a low-toxicity catalytic system for the photopolymerization of polyacrylamide gels. To study the suitability of the proposed system for electrophoretic separation of blood proteins. Object and methods: The samples of donors peripheral blood has been studed. Photopolymerization of acrylamide/N,N′-methylenebisacrylamide was assisted with combination of ethylenediaminetetraacetic acid disodium dihydrate catalyst, riboflavin photoinitiator, and artificial daylight (wavelength range of λ=400–700 nm). Results: a new catalytic system for photopolymerization of polyacrylamide gels was created. The suitability of the proposed method for electrophoretic separation of blood proteins is shown. Conclusions: the use of the proposed method can be particularly appropriate for the study of molecular heterogeneity of enzymes, which can provide additional data on the course and prognosis of cancer.