PROLIFERATIVE ACTIVITY AND ADHESIVE PROPERTIES OF PCa WITH PHENOTYPE OF CANCER STEM CELLS
- 1RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the NASU, Kyiv, Ukraine
- 2National Cancer Institute, Kyiv, Ukraine
Aim: to investigate the proliferative activity and adhesive properties of prostate cancer (PCa) samples with the phenotype of cancer stem cells (CSC). Object and methods: the study was conducted on the clinical material of 120 patients with stage II-IV PCa who were treated at the National Cancer Institute, Ministry of Health of Ukraine during 2015–2017. The study of proliferation, adhesion, and CSC markers expression was performed on FFPE using immunohistochemical methods of mono- and double immunostaining. Analysis of functional interactions of proteins was performed using the resource STRING v.11.0. Statistical processing of the obtained results was performed using Statista 6.0 software. Results: we showed that the presence of CSC in the tumor tissue of PCa patients is associated with high proliferative activity of tumor cells and violation of their adhesive properties by reducing the expression of E-cadherin and increasing the level of N-cadherin. It was determined that high proliferative activity and violation of adhesive properties are features of low-differentiated PCa with the CSC phenotype in patients at late stages of the tumor process, category T3, with the presence of metastatic lesions of regional lymph nodes. We have established an association of the Ki-67 and N-cadherin expression with the Gleason index indicates the participation of these proteins in the formation of the PCa malignancy degree with the expression of CSC markers. Conclusions: thus, the presence of cells with the CSC phenotype against the background of high proliferative potential and violation of intercellular adhesion in PCa tissue is associated with an unfavorable course, which according to clinical and pathological indicators has a high malignancy degree.