PROGNOSTIC MOLECULAR-BIOLOGICAL MARKERS OF MALIGNANT FIBROUS HISTIOCYTOMA
Malignant fibrous histiocytoma (MFH) is one of the most common tumors of soft tissues in adults. The variation in the course of MFH requires the search for additional markers associated with the development of relapses of this nosological entity of oncopathology. Aim: to investigate the molecular-biological and epigenetic features of primary and recurrent MFH. Materials and methods: a retrospective study was conducted on the clinical material of 46 patients with MFH (TIIbN0M0; stage II) with a verified pathomorphological diagnosis of MFH. The content of DNA in tumor cells was determined by flow cytometry, the content in the tumor cells of the proliferation marker KI-67 was determined by immunohistochemical method, the content in the cells of miR-182, miR-199a and miR-34a was determined by reverse transcription polymerase chain reaction. Results: characteristic of molecular-biological signs and epigenetic features of primary and recurrent MFH were studied. It has been found that recurrent MFH is a heterogeneous neoplasm group that contains both diploid and aneuploid tumor types and is characterized by high proliferative activity. The association of the expressions of miR-34a, -182 and 34a, -182 and -199a with the degree of differentiation and proliferative activity of MFH has been proved. Conclusions: the findings are important for understanding the malignant phenotype of MFH and can also be used to develop a marker panel to predict the aggressiveness of the MFH flow. Based on the obtained data, an algorithm for predicting the aggressiveness of the flow of MFH has been developed.