REPLICATIVE STRESS PROTEINS AS POTENTIAL PROGNOSTIC MARKERS OF RECURRENCE OF ENDOMETRIOID CARCINOMA OF THE ENDOMETRIUM IN PATIENTS WITH I DISEASE STAGE
Buchynska L.G., Iurchenko N.P., Nesina I.P., Glushchenko N.M.
- RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, NAS of Ukraine, Kyiv, Ukraine
Aim: on the basis of bioinformatic analysis of the expression of markers associated with replicative stress, to evaluate their significance in the progression of endometrioid carcinoma of the endometrium (ЕСЕ). Object and methods: the study of ATR, ATM, CHEK1/2, FANCD2, BRCA1 gene expression was carried out using the GEPIA web tool. Bioinformatics databases STRING v.11.5 and FunCoup v.5.0 were used for prognostic modeling of the intergenic interaction of the studied genes. Results: interactive analysis of the gene expression profile in both ЕСЕ and normal tissues revealed that the expression of CHEK1/2, FANCD2, and BRCA1 was higher in ЕСЕ compared to normal tissues. However, the expression of ATR and ATM was significantly lower in ЕСЕ than in the alternative group, which may indicate a malfunction of these genes. Potentially significant biomarkers of genome instability associated with replicative stress ATR, ATM, CHEK1/2, FANCD2 and BRCA1 were determined, reflecting such biological processes as DNA replication and repair. Conclusion: the established highly predictive associations between the studied biomarkers ATM, ATR, CHEK1/2, FANCD2, BRCA1 provide the basis for their further evaluation as informative indicators associated with replicative stress in the progression of endometrioid carcinoma of the endometrium of patients with the I stage of the tumor process.
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