THE LEVEL OF MITOCHONDRIAL TRANSMEMBRANE POTENTIAL AND INTENSITY OF FREE RADICAL COMPOUNDS FORMATION IN LYMPHOCYTES OF ENDOMETRICS CANCER PATIENTS
- R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of NAS of Ukraine, Kyiv, Ukraine
The level of the mitochondrial membrane polarization and the intensity of reactive oxygen and nitrogen species are important indicators that reflect changes in the functioning of non-malignant cells from the tumor environment, in particular when radiation therapy and substances with possible radioprotective effect were using. Aim: to investigate the relationship between the level of mitochondrial transmembrane potential (TMP) and the intensity of free radical compounds (FR) formation in peripheral blood lymphocytes (PBL) of patients with endometrial cancer (EC) before and after brachytherapy. Assess the effect of metformin (MF) on these indicators in ex vivo system. Object and methods: in the study used samples of peripheral blood of PE patients before radiation therapy and after the first session of brachytherapy at a dose of 6 Gy, as well as the blood of apparently healthy individuals (control group). MF was added to blood samples at final concentrations of 2 and 20 mM before 1 h of PBL isolation. In the PBL were determined the level of TMP using the dye JC-1 and the intensity of FR formation using the dye DCFH-DA. Results: it is shown that in the PBL of the examined group of RE patients there was a tendency to increase the level of TMP (1,24 times) and decrease the production of FR (1,58 times). Also, in the examined patients with T2–T4 disease stage, the level of TMP and the intensity of FR production was 1,70 and 1,61 times higher, respectively, compared with patients with T1 stage. In contrast to women in the control group, the examined patients showed a significant correlation between the level of TMP and the intensity of FR production (r = 0,428), which was closer after the first session of brachytherapy (r = 0,615). The use of MF, especially at a concentration of 20 mM, reduced the correlation between these indicators. Conclusions: in patients with PE there is a significant correlation between the level of TMP and the FR formation in the PBL. At the beginning of brachytherapy, this correlation becomes closer, which indicates the possibility of using the TMP to predict the occurrence of oxidative stress at the beginning of therapeutic exposure of patients with PE. In the model system, it is shown that MF reduces the relationship between the FR production and the level of TMP in the PBL.