STUDY ON RELATIVE GENE EXPRESSION LEVELS IN TUMOR TISSUE AND IN BLOOD SERUM OF CANCER PATIENTS

Kovalevska L.M.1, Zadvornyi T.V.1, Malysheva T.A.2, Kalman S.S.1, Lukianova N.Yu.1, Kashuba E.V.1

Aim: to develop a method to assess relative gene expression at a level of extracellular RNA (exRNA) isolated from blood serum and total RNA obtained from tumor tissue samples, as well as at the protein level in malignant neoplasms. Object and methods: samples of blood serum and surgical material of 5 patients with brain glioblastomas and blood sera of 4 healthy donors. MRPS18-1 and MRPS18-2 proteins were determined by immunohistochemistry. Statistical analysis was performed, using GraphPadPrism9. Results: the MRPS18-2 gene shows a significant difference in extracellular mRNA levels in patients with brain gliomas and healthy donors. The expression of the MRPS18-2 gene at the mRNA level is not much different in blood serum and tumor tissue, that makes the MRPS18-2 gene a putative tumor marker. Conclusions: our data show the possibility of isolating a sufficient amount of extracellular mRNA from the blood serum of cancer patients for detection by qPCR. In addition to this pilot study, more patients, as well as samples from patients with various nosological forms, such as prostate and breast cancer, should be studied, to deve­lop a panel of non-invasive molecular markers for cancer diagnosis and/or prognosis.



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